USP7 delta C-terminus
(Plasmid
#234074)
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PurposeExpresses USP7 mutant in E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a-LIC
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Backbone manufacturerStructural Genomics Consortium, Toronto
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 8618
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUSP7 1-1080
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Alt nameUbiquitin carboxyl-terminal hydrolase 7
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SpeciesH. sapiens (human)
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Insert Size (bp)3306
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MutationE547 mutated to stop codon
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Entrez GeneUSP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
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Tag
/ Fusion Protein
- 6xHis cleavable with thrombin (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
USP7 delta C-terminus was a gift from Irina Bezsonova (Addgene plasmid # 234074 ; http://n2t.net/addgene:234074 ; RRID:Addgene_234074)