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pLenti_PEmax-2A-MLH1dn-2A-BSD
(Plasmid #234072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-PE2-BSD
  • Backbone manufacturer
    Hyongbum Kim, Addgene plasmid #161514
  • Backbone size w/o insert (bp) 8693
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEmax-2A-MLH1dn
  • Species
    Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV-RT is from the Moloney murine leukemia virus; MLH1dn is from Homo sapiens
  • Insert Size (bp)
    8715
  • Promoter EF-1α core promoter
  • Tags / Fusion Proteins
    • SV40 bpNLS (N terminal on insert)
    • SV40 bpNLS (C terminal on insert)
    • c-Myc NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Liu (pCMV-PEmax-P2A-hMLH1dn, Addgene plasmid #174828)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.01.587366 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_PEmax-2A-MLH1dn-2A-BSD was a gift from Greg Findlay (Addgene plasmid # 234072 ; http://n2t.net/addgene:234072 ; RRID:Addgene_234072)

  • For your References section:

    High-throughput screening of human genetic variants by pooled prime editing. Herger M, Kajba CM, Buckley M, Cunha A, Strom M, Findlay GM. Cell Genom. 2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814. 10.1016/j.xgen.2025.100814 PubMed 40120586
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