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pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro
(Plasmid #234070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-Puro
  • Backbone size (bp) 8856
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

User-specified (e)pegRNA can be inserted downstream of mU6 promoter after BsmBI-digestion of this plasmid. Additionally encodes an epegRNA under hU6 promoter to install ATP1A1-T804N mutation enabling ouabain co-selection.

Please visit https://doi.org/10.1101/2024.04.01.587366 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro was a gift from Greg Findlay (Addgene plasmid # 234070 ; http://n2t.net/addgene:234070 ; RRID:Addgene_234070)

  • For your References section:

    High-throughput screening of human genetic variants by pooled prime editing. Herger M, Kajba CM, Buckley M, Cunha A, Strom M, Findlay GM. Cell Genom. 2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814. 10.1016/j.xgen.2025.100814 PubMed 40120586
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