pDest17_N-myc
(Plasmid
#234045)
-
PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 234045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDest17
-
Backbone manufacturerInvtirogen
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsUse BL21_DE3 pLysS for protein expression
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameN-myc
-
SpeciesH. sapiens (human)
-
Entrez GeneMYCN (a.k.a. FGLDS1, MODED, MPAPA, MYCNsORF, MYCNsPEP, N-myc, NMYC, ODED, bHLHe37)
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDest17_N-myc was a gift from Tanja Mittag (Addgene plasmid # 234045 ; http://n2t.net/addgene:234045 ; RRID:Addgene_234045) -
For your References section:
MYC phase separation selectively modulates the transcriptome. Yang J, Chung CI, Koach J, Liu H, Navalkar A, He H, Ma Z, Zhao Q, Yang X, He L, Mittag T, Shen Y, Weiss WA, Shu X. Nat Struct Mol Biol. 2024 Oct;31(10):1567-1579. doi: 10.1038/s41594-024-01322-6. Epub 2024 May 29. 10.1038/s41594-024-01322-6 PubMed 38811792