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Addgene

pLenti-CR033
(Plasmid #234042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-CR033
  • Total vector size (bp) 13278
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TadA8e(V106W)-Cas9(D10A)-P2A-blast
  • Species
    Streptococcus pyogenes for Cas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CR033 was a gift from Alexander Marson (Addgene plasmid # 234042 ; http://n2t.net/addgene:234042 ; RRID:Addgene_234042)

  • For your References section:

    Base-editing mutagenesis maps alleles to tune human T cell functions. Schmidt R, Ward CC, Dajani R, Armour-Garb Z, Ota M, Allain V, Hernandez R, Layeghi M, Xing G, Goudy L, Dorovskyi D, Wang C, Chen YY, Ye CJ, Shy BR, Gilbert LA, Eyquem J, Pritchard JK, Dodgson SE, Marson A. Nature. 2024 Jan;625(7996):805-812. doi: 10.1038/s41586-023-06835-6. Epub 2023 Dec 13. 10.1038/s41586-023-06835-6 PubMed 38093011
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