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makeTCR_Hs.TRAV12-3
(Plasmid #233954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmakeTCR
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 1710
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAV12-3
  • Alt name
    ENSG00000211794
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    334
  • Entrez Gene
    TRAV12-3 (a.k.a. TCRAV12S3, TCRAV2S2, TRAV123)
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site GATG (not destroyed)
  • 3′ cloning site CTGT (not destroyed)
  • 5′ sequencing primer M13 (-21) Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    makeTCR_Hs.TRAV12-3 was a gift from Michael Platten (Addgene plasmid # 233954 ; http://n2t.net/addgene:233954 ; RRID:Addgene_233954)
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