Addgene: RMCE ABCDE>ala DNA-PKcs Skip to main content
Addgene

RMCE ABCDE>ala DNA-PKcs
(Plasmid #233914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psm7
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human DNA-PKcs ABCDE phosphorylation site mutant
  • Species
    H. sapiens (human)
  • Mutation
    phosphorylation sites 2609, 2612, 2620, 2624, 2638, 2647 alanine substituted
  • Entrez Gene
    PRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RMCE ABCDE>ala DNA-PKcs was a gift from Katheryn Meek (Addgene plasmid # 233914 ; http://n2t.net/addgene:233914 ; RRID:Addgene_233914)

  • For your References section:

    Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining. Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, Chaplin AK, Meek K. Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31. 10.1016/j.molcel.2023.01.012 PubMed 36724784
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