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Addgene

pET-His-E1GFP
(Plasmid #233885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E1GFP
  • Mutation
    T65S and T203Y compared to EGFP
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-4968583/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-His-E1GFP was a gift from Francesco Cardarelli (Addgene plasmid # 233885 ; http://n2t.net/addgene:233885 ; RRID:Addgene_233885)

  • For your References section:

    Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor. De Lorenzi V, Ghignoli S, Bernardi M, Matteoli G, Ferri G, Storti B, Bizzarri R, Cardarelli F. Commun Biol. 2025 Feb 25;8(1):304. doi: 10.1038/s42003-025-07758-w. 10.1038/s42003-025-07758-w PubMed 40000885
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