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Addgene

pCpep-E1GFP
(Plasmid #233884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Proinsulin-E1GFP
  • Species
    M. musculus (mouse)
  • Mutation
    Inserted E1GFP into C-peptide of mouse Ins2 coding sequence. E1GFP harbous T65S and T203Y mutation compared to EGFP
  • Entrez Gene
    Ins2 (a.k.a. Ins-2, InsII, Mody, Mody4)
  • Promoter CMVF

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Cpep-EGFP plasmid on which we have performed site-directed mutagenesis was a gift from prof. David Piston.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-4968583/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCpep-E1GFP was a gift from Francesco Cardarelli (Addgene plasmid # 233884 ; http://n2t.net/addgene:233884 ; RRID:Addgene_233884)

  • For your References section:

    Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor. De Lorenzi V, Ghignoli S, Bernardi M, Matteoli G, Ferri G, Storti B, Bizzarri R, Cardarelli F. Commun Biol. 2025 Feb 25;8(1):304. doi: 10.1038/s42003-025-07758-w. 10.1038/s42003-025-07758-w PubMed 40000885
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