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Addgene

HIS-HA-CMYC P53
(Plasmid #233738)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233738 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 Sumo TEV LIC cloning vector (2S-T)
  • Backbone manufacturer
    Scott Gradia (Addgene plasmid # 29711)
  • Backbone size w/o insert (bp) 5101
  • Total vector size (bp) 6027
  • Modifications to backbone
    removed SUMO and TEV site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIS-HA-CMYC-p53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1314
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS-HA-CMYC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer taatacgactcactatagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIS-HA-CMYC P53 was a gift from Albert Keung (Addgene plasmid # 233738 ; http://n2t.net/addgene:233738 ; RRID:Addgene_233738)

  • For your References section:

    A high sensitivity assay of UBE3A ubiquitin ligase activity. Han L, Begum Yagci Z, Keung AJ. Methods. 2025 Mar;235:92-99. doi: 10.1016/j.ymeth.2025.02.002. Epub 2025 Feb 9. 10.1016/j.ymeth.2025.02.002 PubMed 39933617
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