pOPTG-Tau-RING (M72E/I18R)
(Plasmid
#233692)
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PurposeExpression of Tau-RING I18R-M72E mutant in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOPTG
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTau protein fused to TRIM21 RING domain
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPTG-Tau-RING (M72E/I18R) was a gift from Leo James (Addgene plasmid # 233692 ; http://n2t.net/addgene:233692 ; RRID:Addgene_233692) -
For your References section:
Co-opting templated aggregation to degrade pathogenic tau assemblies and improve motor function. Miller LVC, Papa G, Vaysburd M, Cheng S, Sweeney PW, Smith A, Franco C, Katsinelos T, Huang M, Sanford SAI, Benn J, Farnsworth J, Higginson K, Joyner H, McEwan WA, James LC. Cell. 2024 Oct 17;187(21):5967-5980.e17. doi: 10.1016/j.cell.2024.08.024. Epub 2024 Sep 13. 10.1016/j.cell.2024.08.024 PubMed 39276772