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Addgene

pTon1(acgfp1)
(Plasmid #233648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE-tight
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5542
  • Vector type
    Amphibian Expression, Tet-on

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    23

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTon1(acgfp1) was a gift from Takeo Kubo (Addgene plasmid # 233648 ; http://n2t.net/addgene:233648 ; RRID:Addgene_233648)

  • For your References section:

    Interleukin-11 induces and maintains progenitors of different cell lineages during Xenopus tadpole tail regeneration. Tsujioka H, Kunieda T, Katou Y, Shirahige K, Fukazawa T, Kubo T. Nat Commun. 2017 Sep 8;8(1):495. doi: 10.1038/s41467-017-00594-5. 10.1038/s41467-017-00594-5 PubMed 28887447
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