psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL
(Plasmid
#233628)
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PurposeExpression of bicistronic reporter with TOP motif
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsiCHECK2
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePromoter and 5'UTR of Eef2 gene and HCV IRES
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SpeciesM. musculus (mouse)
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Entrez GeneEef2 (a.k.a. Ef-2)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL was a gift from Shintaro Iwasaki (Addgene plasmid # 233628 ; http://n2t.net/addgene:233628 ; RRID:Addgene_233628) -
For your References section:
eIF4A1 enhances LARP1-mediated translational repression during mTORC1 inhibition. Shichino Y, Yamaguchi T, Kashiwagi K, Mito M, Takahashi M, Ito T, Ingolia NT, Kuba K, Iwasaki S. Nat Struct Mol Biol. 2024 Oct;31(10):1557-1566. doi: 10.1038/s41594-024-01321-7. Epub 2024 May 21. 10.1038/s41594-024-01321-7 PubMed 38773334