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pColdI-DDX3_helicase_core_Q360L
(Plasmid #233619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pColdI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX3X helicase core Gln360Leu
  • Species
    H. sapiens (human)
  • Mutation
    Gln360Leu
  • Entrez Gene
    DDX3X (a.k.a. CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB)
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pColdI-DDX3_helicase_core_Q360L was a gift from Shintaro Iwasaki (Addgene plasmid # 233619 ; http://n2t.net/addgene:233619 ; RRID:Addgene_233619)

  • For your References section:

    Dual targeting of DDX3 and eIF4A by the translation inhibitor rocaglamide A. Chen M, Asanuma M, Takahashi M, Shichino Y, Mito M, Fujiwara K, Saito H, Floor SN, Ingolia NT, Sodeoka M, Dodo K, Ito T, Iwasaki S. Cell Chem Biol. 2021 Apr 15;28(4):475-486.e8. doi: 10.1016/j.chembiol.2020.11.008. Epub 2020 Dec 8. 10.1016/j.chembiol.2020.11.008 PubMed 33296667
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