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Addgene

pcDNA3-hRPL3-FLAG_WT
(Plasmid #233616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-tagged hRPL3
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hRPL3-FLAG_WT was a gift from Shintaro Iwasaki (Addgene plasmid # 233616 ; http://n2t.net/addgene:233616 ; RRID:Addgene_233616)

  • For your References section:

    METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. Matsuura-Suzuki E, Shimazu T, Takahashi M, Kotoshiba K, Suzuki T, Kashiwagi K, Sohtome Y, Akakabe M, Sodeoka M, Dohmae N, Ito T, Shinkai Y, Iwasaki S. Elife. 2022 Jun 8;11. pii: 72780. doi: 10.7554/eLife.72780. 10.7554/eLife.72780 PubMed 35674491
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