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Addgene

pC016-gPsp-EGFP_start_codon
(Plasmid #233613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC016
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting EGFP
  • gRNA/shRNA sequence
    TGCTCACCATGGTAGCTTAAGTT
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC016-gPsp-EGFP_start_codon was a gift from Shintaro Iwasaki (Addgene plasmid # 233613 ; http://n2t.net/addgene:233613 ; RRID:Addgene_233613)

  • For your References section:

    dCas13-mediated translational repression for accurate gene silencing in mammalian cells. Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S. Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7. 10.1038/s41467-024-46412-7 PubMed 38467613
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