Skip to main content
Addgene

pCAGEN-dPspCas13b-NES-mCherry
(Plasmid #233609)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCAGEN
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dPspCas13b-NES-mCherry
  • Species
    Prevotella sp. P5-125
  • Mutation
    H133A/H1058A
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGEN-dPspCas13b-NES-mCherry was a gift from Shintaro Iwasaki (Addgene plasmid # 233609 ; http://n2t.net/addgene:233609 ; RRID:Addgene_233609)

  • For your References section:

    dCas13-mediated translational repression for accurate gene silencing in mammalian cells. Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S. Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7. 10.1038/s41467-024-46412-7 PubMed 38467613
Related items: