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Addgene

ER-RspA-CFAST
(Plasmid #233607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RspA-CFAST
  • Species
    O. cuniculus (rabbit); Other
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-RspA-CFAST was a gift from Riccardo Filadi (Addgene plasmid # 233607 ; http://n2t.net/addgene:233607 ; RRID:Addgene_233607)

  • For your References section:

    Simultaneous detection of membrane contact dynamics and associated Ca(2+) signals by reversible chemogenetic reporters. Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML, Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R. Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0. 10.1038/s41467-024-52985-0 PubMed 39532847
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