Addgene: pCCI-YFVΔCMENS1-Flpo Skip to main content
Addgene

pCCI-YFVΔCMENS1-Flpo
(Plasmid #233592)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 233592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCCI
  • Backbone size w/o insert (bp) 8888
  • Vector type
    Transneural tracing

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TransforMax EPI300
  • Growth instructions
    Grow in LB + chloramphenicol overnight. Follow protocol listed under Supplemental Documents.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YFV-17D lacking C, prM, E and NS1 proteins but with Flpo
  • Species
    Synthetic
  • Mutation
    Lacking C, prM, E and NS1
  • Promoter SP6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please view the attached protocol for growing plasmid in the lab. If protocol is not followed, it is likely DNA preps will contain a significant number of DNA impurities.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCI-YFVΔCMENS1-Flpo was a gift from Wei Xu (Addgene plasmid # 233592 ; http://n2t.net/addgene:233592 ; RRID:Addgene_233592)
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