JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)
(Plasmid
#233545)
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PurposeA CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6.2-V5-DEST
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAFB
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SpeciesH. sapiens (human)
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Entrez GeneMAFB (a.k.a. DURS3, KRML, MCTO)
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Tag
/ Fusion Protein
- V5 (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.15.468651 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry) was a gift from Joshua Wythe (Addgene plasmid # 233545 ; http://n2t.net/addgene:233545 ; RRID:Addgene_233545) -
For your References section:
Defining the Transcriptional and Epigenetic Basis of Organotypic Endothelial Diversity in the Developing and Adult Mouse. Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD. bioRxiv 2021.11.15.468651 10.1101/2021.11.15.468651