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pGADT7_Sr62NLRa
(Plasmid #233528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADT7
  • Backbone manufacturer
    Clontech, PT3249-5
  • Backbone size w/o insert (bp) 7965
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sr62NLRa
  • Species
    Aegilops sharonensis
  • Insert Size (bp)
    3123
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4 AD, HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GAL4 transcriptional activation domain forward
  • 3′ sequencing primer GAL4 transcriptional activation domain reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.30.610287 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7_Sr62NLRa was a gift from Brande Wulff (Addgene plasmid # 233528 ; http://n2t.net/addgene:233528 ; RRID:Addgene_233528)

  • For your References section:

    A wheat tandem kinase sensor activates an NLR helper to trigger immunity. Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł, Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, Wulff BBH. bioRxiv 2024.08.30.610287 10.1101/2024.08.30.610287
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