mito-PAGFP
(Plasmid #23348)

• Purpose: Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.
• Depositing Lab: Richard Youle
• Publication: Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

Backbone

• Vector backbone: mito-GFP (BD Biosciences)
• Backbone size w/o insert (bp): 4059
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAGFP
• Alt name: Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase
• Alt name: photoactivatable mito-GFP
• Insert Size (bp): 741
• Tag / Fusion Protein:
  • mito (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PAGFP-N1, provided by G. Patterson and J. Lippincott-Schwartz, was used as a template for cloning the PAGFP region in place of WT GFP in the mito-GFP vector.
• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry