AAV-SYN-Myc-ATL1-oPRE
(Plasmid
#233471)
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PurposeAAV vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV-SYN
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Backbone manufacturerVectorBuilder
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATL1
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Alt nameAtlastin GTPase 1
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Alt nameSPG3A
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SpeciesH. sapiens (human)
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Insert Size (bp)1710
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Entrez GeneATL1 (a.k.a. AD-FSP, FSP1, GBP3, HSN1D, SPG3, SPG3A, atlastin1)
- Promoter Human synapsin promoter
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Tag
/ Fusion Protein
- myc-tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-SYN-Myc-ATL1-oPRE was a gift from Richard Eva & Bart Nieuwenhuis & Evan Reid (Addgene plasmid # 233471 ; http://n2t.net/addgene:233471 ; RRID:Addgene_233471)
