LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)
(Plasmid
#233427)
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PurposePiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac vector
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Vector typeMammalian Expression
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Selectable markersHygromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLIDARsensor(mcherry-BFP)
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SpeciesSynthetic
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MutationWT
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
https://benchling.com/s/seq-q8G1CIjls4Ajf7wG7vh8?m=slm-kCOlBe1kTC76JYbgcXfg for annotated sequence file. Please visit https://doi.org/10.1101/2024.05.03.592453 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR) was a gift from Xiaojing Gao (Addgene plasmid # 233427 ; http://n2t.net/addgene:233427 ; RRID:Addgene_233427) -
For your References section:
Post-transcriptional modular synthetic receptors. Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu Y, Xie Y, Gao XJ. Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w. 10.1038/s41589-025-01872-w PubMed 40155716
