XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3
(Plasmid
#233420)
-
PurposeIVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233420 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneFLP-IN
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameT7-LIDARsensor-hybridUTR3
-
SpeciesSynthetic
-
MutationWT
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
https://benchling.com/s/seq-K1euM67vxduUsiTwzMAH?m=slm-OlPsGDFUzFqCJ6NpoLwu for annotated sequence file. Please visit https://doi.org/10.1101/2024.05.03.592453 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3 was a gift from Xiaojing Gao (Addgene plasmid # 233420 ; http://n2t.net/addgene:233420 ; RRID:Addgene_233420) -
For your References section:
Post-transcriptional modular synthetic receptors. Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu Y, Xie Y, Gao XJ. Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w. 10.1038/s41589-025-01872-w PubMed 40155716
