pMPMA3dPlac-PfliC-GFPLVA
(Plasmid
#23342)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMPMA3
- Backbone size w/o insert (bp) 3174
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsDH10B Overnight shaking culture in LB broth
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP(LVA)
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SpeciesSalmonella enterica and Aequorea victoria
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Insert Size (bp)1093
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Tag
/ Fusion Protein
- GFP(LVA) (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPMA3dPlac-PfliC-GFPLVA was a gift from Olivia Steele-Mortimer (Addgene plasmid # 23342 ; http://n2t.net/addgene:23342 ; RRID:Addgene_23342) -
For your References section:
Induction of Salmonella Pathogenicity Island 1 under different growth conditions can affect Salmonella-host cell interactions in vitro. Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, Fischer ER, Porcella SF, Steele-Mortimer O. Microbiology. 2009 Dec 24. ():. 10.1099/mic.0.032896-0 PubMed 20035008
