XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461)
(Plasmid
#233414)
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PurposeLIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFLP-IN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-SGc(stem-1(3xMS2))cSG-TEVP
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SpeciesSynthetic
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MutationWT
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
https://benchling.com/s/seq-JjXSoNTmFcjKrHCszCQM?m=slm-r7r8gX9iR7EuzVdWPyd5 for annotated sequence file. Please visit https://doi.org/10.1101/2024.05.03.592453 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461) was a gift from Xiaojing Gao (Addgene plasmid # 233414 ; http://n2t.net/addgene:233414 ; RRID:Addgene_233414) -
For your References section:
Post-transcriptional modular synthetic receptors. Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu Y, Xie Y, Gao XJ. Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w. 10.1038/s41589-025-01872-w PubMed 40155716