Skip to main content
Addgene

XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export
(Plasmid #233385)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PiggyBac vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin ; TagBFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VEGFR1(ecto+TM)-ADAR2dd
  • Alt name
    VEGFR2(ecto+TM)-tdMCP
  • Species
    Synthetic
  • Mutation
    E488Q, T501A on ADAR2dd
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG, HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export was a gift from Xiaojing Gao (Addgene plasmid # 233385 ; http://n2t.net/addgene:233385 ; RRID:Addgene_233385)

  • For your References section:

    Post-transcriptional modular synthetic receptors. Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu Y, Xie Y, Gao XJ. Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w. 10.1038/s41589-025-01872-w PubMed 40155716
Related items: