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pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
(Plasmid #233369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7709
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-Kinprola_PKA-mEGFP
  • Species
    Synthetic
  • Insert Size (bp)
    2277
  • Promoter CMV
  • Tags / Fusion Proteins
    • NES (N terminal on insert)
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.11.611894 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP was a gift from Kai Johnsson (Addgene plasmid # 233369 ; http://n2t.net/addgene:233369 ; RRID:Addgene_233369)

  • For your References section:

    Molecular recording of cellular protein kinase activity with chemical labeling. Sun D, Wang Ng S, Zheng Y, Xie S, Schwan N, Breuer P, Hoffmann DC, Michel J, Azorin DD, Boonekamp KE, Winkler F, Wick W, Boutros M, Li Y, Johnsson K. bioRxiv 2024.09.11.611894 10.1101/2024.09.11.611894
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