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Addgene

Sox17 Flox
(Plasmid #23335)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ploxPFLPneo
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sex determining region Y-box containing gene 17
  • Alt name
    Sox17
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4299
  • Mutation
    None
  • GenBank ID
    NM_011441
  • Entrez Gene
    Sox17

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Hinc2 (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The source of ploxPFLPneo is
Hiraoka et al Mol Cell Biol 26, 6139.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sox17 Flox was a gift from Sean Morrison (Addgene plasmid # 23335 ; http://n2t.net/addgene:23335 ; RRID:Addgene_23335)

  • For your References section:

    Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells. Kim I, Saunders TL, Morrison SJ. Cell. 2007 Aug 10. 130(3):470-83. 10.1016/j.cell.2007.06.011 PubMed 17655922
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