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pMRX-IZ-SEC61B-mCherry-FRB
(Plasmid #233349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IZ
  • Total vector size (bp) 7163
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    DH5alpha will also be fine
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEC61B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    288
  • Entrez Gene
    SEC61B

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    705

Gene/Insert 3

  • Gene/Insert name
    FRB
  • Mutation
    T2098L mutant of FKBP-rapamycin binding domain of human FRAP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.13.607738 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IZ-SEC61B-mCherry-FRB was a gift from Noboru Mizushima (Addgene plasmid # 233349 ; http://n2t.net/addgene:233349 ; RRID:Addgene_233349)

  • For your References section:

    Optogenetic tools for inducing organelle membrane rupture. Nagashima Y, Eguchi T, Koyama-Honda I, Mizushima N. J Biol Chem. 2025 Mar 18;301(4):108421. doi: 10.1016/j.jbc.2025.108421. 10.1016/j.jbc.2025.108421 PubMed 40113041
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