Sox17 GFP
(Plasmid
#23334)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneploxPFLPneo
- Backbone size w/o insert (bp) 3000
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSex determining region Y-box containing gene 17
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SpeciesM. musculus (mouse)
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Insert Size (bp)4299
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MutationNone
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GenBank IDNM_011441
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Entrez GeneSox17
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Hinc2 (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The source of ploxPFLPneo is Hiraoka et al Mol Cell Biol 26, 6139.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sox17 GFP was a gift from Sean Morrison (Addgene plasmid # 23334 ; http://n2t.net/addgene:23334 ; RRID:Addgene_23334) -
For your References section:
Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells. Kim I, Saunders TL, Morrison SJ. Cell. 2007 Aug 10. 130(3):470-83. 10.1016/j.cell.2007.06.011 PubMed 17655922
Map uploaded by the depositor.
