DEXsynth:eGFP-ccdb + mcherry
(Plasmid #233311)

• Purpose: GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker
• Depositing Lab: Thomas Baum
• Publication: GATEWAY compatible vector with a promoter driving in frame epitope fusion paired with ccdb selection toxin and FP marker (unpublished)

Backbone

• Vector backbone: pICH75322
• Vector type: Gateway destination

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Tetracycline, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DEXsynth:eGFP-ccdb + mcherry
• Species: Other
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • DEX_eGFP-ccdb + mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.comPlease note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.