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AAV-U6/TO-shEsr1-CMV-TetR-eGFP
(Plasmid #233298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
  • Backbone manufacturer
    Jonathan Ploski (Addgene #82706)
  • Backbone size w/o insert (bp) 6298
  • Total vector size (bp) 6240
  • Modifications to backbone
    Remove the sequence of gRNA scaffold
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Esr1 shRNA
  • Alt name
    ERα
  • gRNA/shRNA sequence
    GGC ATG GAG CAT CTC TAC A
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Esr1 (a.k.a. ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer hU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a D insertion after T52 upstream of TetR and does not affect function. The expression of TetR was confirmed using Western Blotting in in vitro cell culture experiment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-U6/TO-shEsr1-CMV-TetR-eGFP was a gift from Dayu Lin (Addgene plasmid # 233298 ; http://n2t.net/addgene:233298 ; RRID:Addgene_233298)

  • For your References section:

    Estrogenic control of reward prediction errors and reinforcement learning. Golden CEM, Martin AC, Kaur D, Mah A, Levy DH, Yamaguchi T, Lasek AW, Lin D, Aoki C, Constantinople CM. bioRxiv [Preprint]. 2024 Sep 25:2023.12.09.570945. doi: 10.1101/2023.12.09.570945. 10.1101/2023.12.09.570945 PubMed 38105956
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