SRP19 in pLX311
(Plasmid
#233292)
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PurposeExpression of WT SRP19
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX311
- Total vector size (bp) 8657
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRP19
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SpeciesH. sapiens (human)
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Insert Size (bp)462
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Entrez GeneSRP19
- Promoter E1Fa
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TTCTTCCATTTCAGGTGTCGTGAG
- 3′ sequencing primer GTGGATACGCTGCTTTAATGCCTT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.30.578083 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SRP19 in pLX311 was a gift from Sefi Rosenbluh (Addgene plasmid # 233292 ; http://n2t.net/addgene:233292 ; RRID:Addgene_233292)