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inducible shSRP19_9 in pLKO tet on
(Plasmid #233289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO tet on
  • Total vector size (bp) 8816
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shSRP19
  • gRNA/shRNA sequence
    ATGAATGGAGACTTCTAATTT
  • Species
    H. sapiens (human)
  • GenBank ID
    SRP19
  • Promoter Dox

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer CGGTATCGATCACGAGACTAGCC
  • 3′ sequencing primer GACGTGAAGAATGTGCGAGACCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dox inducible expression of SRP19 targeting shRNA. Please visit https://doi.org/10.1101/2024.01.30.578083 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    inducible shSRP19_9 in pLKO tet on was a gift from Sefi Rosenbluh (Addgene plasmid # 233289 ; http://n2t.net/addgene:233289 ; RRID:Addgene_233289)
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