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Addgene

AAV-EF1α-loxP-GCaMP6f-loxP-WPRE
(Plasmid #233288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV-EF1α-loxP-loxP-WPRE
  • Total vector size (bp) 6825
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    1353
  • Promoter EF1α

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer EF1α
  • 3′ sequencing primer WPRE
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-EF1α-loxP-GCaMP6f-loxP-WPRE was a gift from Dayu Lin (Addgene plasmid # 233288 ; http://n2t.net/addgene:233288 ; RRID:Addgene_233288)

  • For your References section:

    A Hypothalamic Midbrain Pathway Essential for Driving Maternal Behaviors. Fang YY, Yamaguchi T, Song SC, Tritsch NX, Lin D. Neuron. 2018 Apr 4;98(1):192-207.e10. doi: 10.1016/j.neuron.2018.02.019. 10.1016/j.neuron.2018.02.019 PubMed 29621487
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