pCARGO-RMCE-Xist
(Plasmid
#233268)
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PurposeCARGO plasmid containing ~22kb dox-inducible Xist transgene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size w/o insert (bp) 2500
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Modifications to backboneContains TRE dox-inducible promoter, CARGO cloning site with Xist gene inserted, and polyA followed by PGK-Hygro without polyA and flanked by FRT sites. The entire series of elements are flanked by LoxP71 and LoxP2272 elements to facilitate directional recombination into LoxP66/2272 sites engineered into the Rosa26 locus.
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name22kb Xist gene, including introns
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SpeciesM. musculus (mouse)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCARGO-RMCE-Xist was a gift from Mauro Calabrese (Addgene plasmid # 233268 ; http://n2t.net/addgene:233268 ; RRID:Addgene_233268)