pOG-Cre
(Plasmid
#233265)
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PurposePlasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOG
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Modifications to backboneCre-expressing plasmid used to perform RMCE with pCARGO-Xist and -Airn
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOG-Cre was a gift from Mauro Calabrese (Addgene plasmid # 233265 ; http://n2t.net/addgene:233265 ; RRID:Addgene_233265) -
For your References section:
Isogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAs. Trotman JB, Abrash EW, Murvin MM, Braceros AK, Li S, Boyson SP, Salcido RT, Cherney RE, Bischoff SR, Kaufmann K, Eberhard QE, Zhang Z, Cowley DO, Calabrese JM. Mol Cell. 2025 Mar 20;85(6):1117-1133.e14. doi: 10.1016/j.molcel.2025.02.014. 10.1016/j.molcel.2025.02.014 PubMed 40118040