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HemH-R212A_pET-14b
(Plasmid #233262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-14b
  • Backbone size w/o insert (bp) 4671
  • Total vector size (bp) 5115
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HemH
  • Insert Size (bp)
    978
  • Mutation
    Arginine was replaced with Alanine at position 212 (R212A)
  • GenBank ID
    NP_415008
  • Entrez Gene
    hemH (a.k.a. b0475, ECK0469, popA, visA)
  • Promoter T7 promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript cloned

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HemH-R212A_pET-14b was a gift from Mohan Babu & Andrew Emili (Addgene plasmid # 233262 ; http://n2t.net/addgene:233262 ; RRID:Addgene_233262)

  • For your References section:

    Ligand interaction landscape of transcription factors and essential enzymes in E. coli. Peng H, Kotelnikov S, Egbert ME, Ofaim S, Stevens GC, Phanse S, Saccon T, Ignatov M, Dutta S, Istace Z, Moutaoufik MT, Aoki H, Kewalramani N, Sun J, Gong Y, Padhorny D, Poda G, Alekseenko A, Porter KA, Jones G, Rodionova I, Guo H, Pogoutse O, Datta S, Saier M, Crovella M, Vajda S, Moreno-Hagelsieb G, Parkinson J, Segre D, Babu M, Kozakov D, Emili A. Cell. 2025 Jan 22:S0092-8674(25)00032-7. doi: 10.1016/j.cell.2025.01.003. 10.1016/j.cell.2025.01.003 PubMed 39862855
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