pET28a-AviTag-CC-S2
(Plasmid
#233243)
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PurposeBacterial expression of Avi-CC-S2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5219
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAviTag-CC-S2
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SpeciesSynthetic
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Insert Size (bp)345
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-AviTag-CC-S2 was a gift from Khalid Salaita (Addgene plasmid # 233243 ; http://n2t.net/addgene:233243 ; RRID:Addgene_233243) -
For your References section:
Digital and Tunable Genetically Encoded Tension Sensors Based on Engineered Coiled-Coils. Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, Salaita K. Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359. 10.1002/anie.202407359 PubMed 39805005