pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt
(Plasmid
#233240)
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PurposeParallel coiled-coil with (GGS)3 linker to prone condensation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5402
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVh-clover-GCN4-(GGS)3-Vt
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SpeciesSynthetic
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Insert Size (bp)4146
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T21
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt was a gift from Khalid Salaita (Addgene plasmid # 233240 ; http://n2t.net/addgene:233240 ; RRID:Addgene_233240) -
For your References section:
Digital and Tunable Genetically Encoded Tension Sensors Based on Engineered Coiled-Coils. Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, Salaita K. Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359. 10.1002/anie.202407359 PubMed 39805005