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Anti-pTau nanobody (A2) with sortase tag
(Plasmid #233218)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 5871
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-pTau nanobody (A2)
  • Alt name
    A2
  • Insert Size (bp)
    465
  • Tag / Fusion Protein
    • Sortase tag, His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer We used whole-plasmid sequencing to confirm insertion
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From the paper: Li T et al., "Camelid single-domain antibodies: A versatile tool for in vivo imaging of extracellular and intracellular brain targets." J Control Release. 2016 Dec 10;243:1-10. doi: 10.1016/j.jconrel.2016.09.019. With permission from the corresponding authors.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.24.563674 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-pTau nanobody (A2) with sortase tag was a gift from Jeff Lichtman (Addgene plasmid # 233218 ; http://n2t.net/addgene:233218 ; RRID:Addgene_233218)

  • For your References section:

    Mapping Alzheimer Disease Molecular Pathologies in Large-Scale Connectomics Data: A Publicly Accessible Correlative Microscopy Resource. Han X, Li PH, Wang S, Blakely T, Aggarwal S, Gopalani B, Sanchez M, Schalek R, Meirovitch Y, Lin Z, Berger D, Wu Y, Aly F, Bay S, Delatour B, Lafaye P, Pfister H, Wei D, Jain V, Ploegh H, Lichtman J. bioRxiv 2023.10.24.563674 10.1101/2023.10.24.563674
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