pLARRYv2-EGFP-T2A-Flpo
(Plasmid
#233212)
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Purpose(Empty Backbone) Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLARRYv2
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Vector typeMammalian Expression, Lentiviral
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.14.594084 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLARRYv2-EGFP-T2A-Flpo was a gift from Alejo Rodriguez-Fraticelli (Addgene plasmid # 233212 ; http://n2t.net/addgene:233212 ; RRID:Addgene_233212) -
For your References section:
Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemic driver mutations. Singh I, Fernandez-Perez D, Sanchez PS, Rodriguez-Fraticelli AE. Cell Stem Cell. 2025 Feb 19:S1934-5909(25)00012-8. doi: 10.1016/j.stem.2025.01.012. 10.1016/j.stem.2025.01.012 PubMed 40010350