pMXs-LIN (with mNgn2x3HA)
(Plasmid
#233194)
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PurposeRetroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs-gw
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-LIN (with mNgn2x3HA) was a gift from Kate Galloway (Addgene plasmid # 233194 ; http://n2t.net/addgene:233194 ; RRID:Addgene_233194) -
For your References section:
Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversion. Wang, Nathan B. et al.. Cell Systems, Volume 0, Issue 0, 101206 10.1016/j.cels.2025.101206