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Addgene

pMXs-LIN (with mNgn2x3HA)
(Plasmid #233194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs-gw
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mLhx3-P2A-Isl1-T2A-mNgn2x3HA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
  • Entrez Gene
    Isl1
  • Entrez Gene
    Lhx3 (a.k.a. Lim3, P-LIM, mLIM3, mLim-3)
  • Tag / Fusion Protein
    • x3HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-LIN (with mNgn2x3HA) was a gift from Kate Galloway (Addgene plasmid # 233194 ; http://n2t.net/addgene:233194 ; RRID:Addgene_233194)

  • For your References section:

    Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversion. Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE. Cell Syst. 2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206. 10.1016/j.cels.2025.101206 PubMed 40086435
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