cASIC1 concatemer - Subunit B
(Plasmid
#233130)
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PurposeTo create cASIC1 channels of defined stoichiometry in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cASIC1 concatemer - Subunit B was a gift from David MacLean (Addgene plasmid # 233130 ; http://n2t.net/addgene:233130 ; RRID:Addgene_233130) -
For your References section:
Proline substitutions in the ASIC1 beta11-12 linker slow desensitization. Purohit R, Couch T, Rook ML, MacLean DM. Biophys J. 2024 Oct 15;123(20):3507-3518. doi: 10.1016/j.bpj.2024.08.016. Epub 2024 Sep 3. 10.1016/j.bpj.2024.08.016 PubMed 39182166