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PX458-eBFP2-sgRNA_CTCF_ZF1
(Plasmid #233090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Backbone manufacturer
    Fheng Zhang (Addgene #48138)
  • Modifications to backbone
    spCas9-T2A-eGFP was modified to spCas9-T2A-eBFP2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spCas9-T2A-eBFP2
  • gRNA/shRNA sequence
    TTTGCAGTTACACATGTCCC
  • Entrez Gene
    Ctcf

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX458-eBFP2-sgRNA_CTCF_ZF1 was a gift from Danny Reinberg (Addgene plasmid # 233090 ; http://n2t.net/addgene:233090 ; RRID:Addgene_233090)

  • For your References section:

    CTCF-RNA interactions orchestrate cell-specific chromatin loop organization. Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D. bioRxiv, March 19, 2025 10.1101/2025.03.19.643339
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