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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G
(Plasmid #233063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5248
  • Total vector size (bp) 10120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADGRL3
  • Alt name
    LPHN3
  • Alt name
    Latrophilin-3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4572
  • Mutation
    Threonine 923 to Glycine
  • Entrez Gene
    Adgrl3 (a.k.a. 5430402I23Rik, CIRL-3, D130075K09Rik, Gm1379, LEC3, Lphn3, mKIAA0768)
  • Tags / Fusion Proteins
    • ALFA (N terminal on insert)
    • HiBiT (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor recommends using NEB Stable cells if experiencing issues with DH5 alpha.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G was a gift from Jonathan Javitch (Addgene plasmid # 233063 ; http://n2t.net/addgene:233063 ; RRID:Addgene_233063)

  • For your References section:

    N-terminal fragment shedding contributes to signaling of the full-length adhesion receptor ADGRL3. Perry-Hauser NA, Du Rand JR, Lee KH, Shi L, Javitch JA. J Biol Chem. 2025 Jan 9:108174. doi: 10.1016/j.jbc.2025.108174. 10.1016/j.jbc.2025.108174 PubMed 39798870
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