pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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> Addgene NGS Result ttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtt tgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaa atactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacct cgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactca agacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgg agcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagg gagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggg ggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgat gctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttg ctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgccttt gagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaag agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtt tcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccag gctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaa acagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgca agcttaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgcct tacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattagg aaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtattt aagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggct aactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtct gttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctga agcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctag aaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattc ggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacg attcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaacca tcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagac caccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtga attatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtg gtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagca ctatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagca gaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcag ctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgct ctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagat ttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactcctta attgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtt tgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggctt ggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccatta tcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggag agagagacagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttttaaaagaaa aggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaa gaattacaaaaacaaattacaaaaattcaaaattttatcgatgcggccgctcggtccagtgaaaaaaatg ctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaactcgagtta cttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcg cgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacgg ggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtg gcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttg tagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgc ggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaa gatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcg gggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagct tgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacac gctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcc tcgcccttgctcaccatggtggcggcgaattcccgcgtcacgacacctgtgttctggcggcaaacccgtt gcgaaaaagaacgttcacggcgactactgcacttatatacggttctcccccaccctcgggaaaaaggcgg agccagtacacgacatcactttcccagtttaccccgcgccaccttctctaggcaccggttcaattgccga cccctccccccaacttctcggggactgtgggcgatgtgcgctctgcccactgacgggcaccggagccact cgaacgcgtatcacgagactagcctcgagttggctttactccctatcagtgatagagaacgtatgaagag tttactccctatcagtgatagagaacgtatgcagactttactccctatcagtgatagagaacgtataagg agtttactccctatcagtgatagagaacgtatgaccagtttactccctatcagtgatagagaacgtatct acagtttactccctatcagtgatagagaacgtatatccagtttactccctatcagtgatagagaacgtat aagctttaggcgtgtacggtgggcgcctataaaagcagagctcgtttagtgaaccgtcagatcgcctgga gcaattccacaacacttttgtcttataccaactttccgtaccacttcctaccctcgtaaaggatccgccg ccaccatgaagcccagcgcagagtgctgttctcccaagttctggttggtgttggccgtcttggccgtatc gggcagcaaagctcgttcccaaaagagcgcccccagcatcggcatcgctgtcatcctcgtgggcacttcc gacgaagtggccataaaagatgccgactacaaggacgacgatgacaagcacgagaaagatgacttccatc atctctcagtagttccccgggtggagctggtagccatgaacgagactgacccaaagagcataatcacccg catctgcgatcttatgtctgaccggaagatccagggggtggtgttcgcggatgacacggaccaggaagcc atcgcccagatcctcgatttcatttctgctcagactctcacccccatcctgggcatccatgggggctcat ctatgataatggcagataaggatgagtcctccatgttcttccagtttggcccatccattgaacagcaagc ttctgtcatgctcaacatcatggaagaatacgactggtacatcttctccatcgtcaccacctacttcccc ggctaccaggacttcgtgaacaagatccgcagcactattgagaacagctttgtaggctgggagctcgagg aagtcctcctgctagacatgtctctagacgatggcgactctaagattcagaatcagctgaagaagctgca gagccccatcattctcctctactgcacaaaggaagaagccacctacatcttcgaagtagctaactcagtt gggctgactggctacggctacacatggatcgtgccgagtctggtggcgggggatacagacacggtgcctt cagagttccccacggggctcatctctgtgtcatatgacgaatgggactatggccttcctgccagagtgag agatgggattgccatcatcaccactgctgcctcggacatgctgtccgaacacagtttcatccctgagccc aagagcagttgctacaacacccacgagaagaggatctaccagtctaacatgctgaataggtatctgatca acgtcacttttgaagggagaaacctgtccttcagtgaagatggctaccagatgcatccgaagctggtgat aatccttctgaacaaggagaggaagtgggagagggtgggaaaatggaaagacaagtccctgcagatgaaa tactacgtgtggcctcgaatgtgtccagagactgaagaacaggaagatgaccatctgagcatcgttacct tggaggaggcaccgtttgtcattgtggaaagtgtggaccctctcagtgggacctgcatgcggaatacagt cccgtgccagaagcgcatcatctctgagaataaaacagatgaggaaccaggctacatcaaaaaatgctgc aaggggttttgtattgatatccttaagaaaatttctaagtctgtgaagttcacctatgacctttacctgg tgaccaatggcaagcatggaaagaaaatcaacgggacctggaacggcatgattggtgaggtggtcatgaa gagggcctacatggcagtgggatcactaactatcaatgaagaacggtcagaggtggttgacttctctgtg cccttcatagagactggcatcagtgtcatggtatcacgcagcaatgggactgtgtcaccttctgccttct tagagccattcagtgctgacgtgtgggtgatgatgtttgtgatgctgctcattgtctctgctgtagctgt ctttgtctttgaatacttcagccctgtgggttacaaccggtgcctagctgatggcagagagccaggcggc ccatctttcaccatcggcaaagcgatttggttactctggggtctggtgtttaacaactccgtacctgtgc agaacccaaaggggaccacctccaagatcatggtgtcagtgtgggccttctttgctgtcattttcctggc cagctacactgccaacttagccgccttcatgatccaagaggagtatgtggaccaggtttccggcctgagt gacaagaagttccagagacctaatgacttctcaccccctttccgctttgggactgtgcccaatggcagca cagagaggaatatccgtaataactatgcagaaatgcatgcctacatgggaaagttcaaccaaaggggtgt agatgatgccttgctctccctgaaaacagggaaacttgatgcattcatctacgatgcagccgtgctcaac tacatggctggaagagacgaaggctgcaagctggtgaccattggcagtggcaaggtctttgcttctacgg gctatggcattgctatccaaaaagactctggttggaaacgccaggtggaccttgctatcctgcagctgtt tggagatggggagatggaagaactggaagctctctggctcactggcatttgccacaatgagaagaatgag gttatgagcagccagctggacattgacaacatggcgggcgtcttctatatgttgggggcagccatggctc tcagcctcatcaccttcatctgtgaacatctcttctattggcagttccgacattgcttcatgggtgtctg ttctggcaagcctggcatggtcttctccatcagcagaggtatctacagctgtatccacggagtagctata gaggagcgccaatccgtgatgaactcccccactgccaccatgaacaacacacactccaatatcctacgct tgctccgaacggccaaaaacatggccaacctgtctggagtcaacggctccccccagagtgccctggactt catccgccgtgagtcctctgtctatgacatctctgagcatcgccgcagcttcacgcattcagactgcaag tcgtacaataacccaccctgtgaggaaaacctgttcagtgactacattagtgaggtagagagaacatttg gcaacctgcagctgaaggacagcaatgtgtaccaagaccactatcaccatcaccaccggccccacagcat cggcagcaccagctccattgatgggctctatgactgtgacaacccaccctttaccacccagcccaggtca atcagcaagaaacccctggacattggcctgccctcctccaaacacagccagctcagcgacctgtacggca agttctctttcaagagtgaccgctacagtggccatgatgacttgattcgatcggatgtctcagacatctc cacgcatactgtcacctatggcaacatcgagggcaacgcagccaagaggaggaagcagcaatataaggac agtctaaagaagcggccagcctcggccaaatctaggagggagtttgatgaaatcgagctggcctaccgtc gccgaccaccccgctccccagaccacaagcgctacttcagggacaaagaagggctccgagacttctacct ggaccagttccgaacaaaggagaactcgcctcactgggagcacgtggacttaactgacatttacaaagaa cgtagtgatgacttcaagcgagattcggtcagtggaggcgggccctgtaccaacaggtctcaccttaaac acggaacaggcgataagcacggagtggtaggcggggtgcctgctccttgggagaagaacctgaccaatgt ggattgggaggataggtctgggggcaacttctgccgcagctgtccctccaagctgcacaattactcctct acggtggcagggcaaaactcgggccggcaggcctgcatcaggtgtgaggcctgcaagaaggctggcaacc tgtatgacatcagcgaggacaactccctgcaggaactggaccagcctgctgcccctgtggctgtgtcatc caacgcctccaccaccaagtatcctcaaagcccgactaattccaaggcccagaagaagaatcggaacaaa ctgcgccggcagcactcctacgacaccttcgtggacctgcagaaggaggaggccgccttggccccacgca gcgtgagcctgaaagacaagggccgattcatggatgggagcccctacgcccatatgtttgagatgccagc tggtgagagctcctttgccaacaagtcctcagtgaccactgccggacaccatcacaacaatcccggcagc ggctacatgctcagcaagtcgctctaccctgaccgggtcacgcaaaaccctttcatccccacttttgggg atgatcagtgcttgcttcacggcagcaaatcctacttcttcaggcagcccacggtggcaggggcgtcgaa aacaaggccggacttccgggcccttgtcaccaataagccagtggtgtcagcccttcatggggctgtgcca ggtcgtttccagaaggacatttgtatagggaaccagtccaacccctgtgtgcctaacaacaaaaacccca gggctttcaatggctccagcaatggacatgtttatgagaaactttctagtattcagtctgatgtctgagt gaggggaattgatccggtacccgcgtaccggttagtaatgatcgacaatcaacctctggattacaaaatt tgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgc ctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtc tctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacc cccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattg ccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaa ttccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctg cgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgc cggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctc cccgcctggcgatggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaa agaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactggg tctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctc aataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatc cctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtat ttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttaca aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtc caaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccatcccgcccc taactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccga ggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagggacgtaccca attcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaa ccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagag gcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcg cattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgc tcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcggggg ctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggtt cacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatag tggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggatt ttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaa tattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttc taaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaa ggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttac atcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatga gcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcg ccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggc atgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctga caacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatg gcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagact ggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctga taaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcc cgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgaga taggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccct taacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttc

pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE Sequences (1)

Full Sequences from Addgene (1)

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