pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA
(Plasmid
#233040)
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PurposeTo express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse homeostatic iron regulator with H67D mutation
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Alt nameHfe
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SpeciesM. musculus (mouse)
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MutationH67D
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Entrez GeneHfe (a.k.a. MR2)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 233040 ; http://n2t.net/addgene:233040 ; RRID:Addgene_233040)